Proceedings of International Conference on Applied Innovation in IT  ·  2026/06/12  ·  Vol. 14  ·  Issue 4  ·  pp. 735–743
Molecular Analysis and Phylogenetic Classification of Uropathogenic Escherichia coli Isolated from Patients with Urinary Tract Infections
Abbas Falih Al-Arnawtee, Ali Jaffar Saleem and Abdel-Raheem Ali
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Abstract
Urinary tract infections are one of the most prevalent bacterial infections all over the world, with Escherichia coli responsible for a large number of clinical cases. Bacterial pathogens employ multiple mechanisms to invade the host. A major mechanism in these strategies is the presence of secretion systems, including secretory system VIII, where the csgG gene encodes the outer membrane channel of the system. Study aimed to consider the importance of the class VIII secretion system in E. coli bacteria for biofilm formation; this study investigates the prevalence of the csgG gene encoding the outer membrane channel of this system. This study involved collecting 378 midstream specimens of urine from patients with urinary tract infections. Some virulence genes were detected and the phylogenetic groups were determined. In addition, detection of the csgG gene. Results show E. coli isolates were identified using an Enterobacteriaceae T-Kit and trpA gene (used as an internal control following the Clermont method). All 82 isolates were confirmed as E. coli. Phylogenetic groups were determined for all isolates: B2, D, A, C, B1, and F, representing 25.61%, 18.29%, 17.07%, 14.63%, 14.63%, and 9.77%, respectively. The present study showed detection of the genes: fimH, papGII, afa and sfa at 82, 79, 20 and 13 isolates, representing 100%, 96.34%, 24.39% and 15.85% respectively. The results also showed that 44 isolates that were selected were all found to contain the csgG gene 100 %. In conclusion, all 44 isolates selected for csgG gene detection carried this gene, indicating their ability to produce Curli fibers via the T8SS. These results indicate a possible association between the presence of the csgG and biofilm production in UPECs, suggesting that the gene plays a pivotal role in adhesion to urinary tract surface structures and in protecting bacteria from host defences.
Keywords
UPEC Virulence Genes Phylogenetic Groups (Clermont) T8SS csgG
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