Proceedings of International Conference on Applied Innovation in IT
2026/03/31, Volume 14, Issue 1, pp.363-369
Computational Data Analysis of Cloud Point Extraction Method for the Determination of Sulfamethoxazole in Pure and Pharmaceutical
Iqbal Salman Mohammed, Noor Ali Hussein Alwan, Sahar Hazbar Haadi and Waseela A. Abdul Razag Abstract: A spectrophotometric method for the determination of sulfamethoxazole (SMZ) was developed using an azo coupling reaction combined with Cloud Point Extraction (CPE) in the presence of the nonionic surfactant Triton X-114. The previously developed azo coupling method demonstrated acceptable accuracy but suffered from limited sensitivity and interference from excipients that affected selectivity. In the present investigation, CPE was employed as a preconcentration step to overcome these limitations, allowing extraction of the colored azo product into the surfactant-rich phase. The method was optimized regarding the surfactant type and concentration, equilibrium temperature, and incubation time. The results showed significant improvement in sensitivity, precision, and accuracy, with a detection wavelength of 489 nm, recoveries around 101.8%, and low relative standard deviations. This technique was successfully applied to pharmaceutical preparations of SMZ without requiring additional complex steps, demonstrating superior sensitivity, selectivity, and environmental compatibility compared with the azo method alone. Thus, CPE provides a simple, cost-effective, and green analytical approach for the determination of sulfamethoxazole in both pure and pharmaceutical forms.
Keywords: Sulfamethoxazole, Cloud Point Extraction, Triton X-114, Spectrophotometry, Pharmaceutical Analysis.
DOI: Under indexing
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References:
- M. Thani, S. Dadoosh, A. Fahad, A. Abdullah, and Y. Fahad, “Synthesis of new azo compound and its application for spectrophotometric determination of sulfamethoxazole and extraction using cloud point extraction,” International Journal of Drug Delivery Technology, vol. 12, no. 3, pp. 1311-1318, 2022.
- N. S. Mohammed, N. Theia’a, and P. Abdul-Jabar, “Development method for spectrophotometric analysis of sulfamethoxazole using vanilline reagent,” Asian Journal of Applied Chemistry Research, vol. 6, no. 5, pp. 41-49, 2020.
- Y. Chu, C. Zhang, R. Wang, X. Chen, N. Ren, and S.-H. Ho, “Biotransformation of sulfamethoxazole by microalgae: removal efficiency, pathways, and mechanisms,” Water Research, vol. 221, p. 118834, 2022.
- D. K. Chellappan, et al., “Exploring the role of antibiotics and steroids in managing respiratory diseases,” Journal of Biochemical and Molecular Toxicology, vol. 36, no. 10, p. e23174, 2022.
- D. Jamil, R. S. AlSayed, and N. K. Abood, “New spectrophotometric determination sulfamethoxazole drug via various analytical methods in pharmaceutical formulation,” Journal of Kufa for Chemical Sciences, vol. 3, no. 1, pp. 197-210, 2023.
- J. G. McCutcheon, A. Lin, and J. J. Dennis, “Characterization of Stenotrophomonas maltophilia phage AXL1 as a member of the genus Pamexvirus encoding resistance to trimethoprim–sulfamethoxazole,” Scientific Reports, vol. 12, no. 1, p. 10299, 2022.
- J. Musial, D. T. Mlynarczyk, and B. J. Stanisz, “Photocatalytic degradation of sulfamethoxazole using TiO2-based materials–Perspectives for the development of a sustainable water treatment technology,” Science of The Total Environment, vol. 856, p. 159122, 2023.
- M. K. Gupta, et al., “A comparative review on high-performance liquid chromatography (HPLC), ultra performance liquid chromatography (UPLC) & high-performance thin layer chromatography (HPTLC) with current updates,” Current Issues in Pharmacy and Medical Sciences, vol. 35, no. 4, pp. 224-228, 2022.
- W. Parys, M. Dołowy, and A. Pyka-Pająk, “Significance of chromatographic techniques in pharmaceutical analysis,” Processes, vol. 10, no. 1, p. 172, 2022.
- A. Radhi, et al., “Instrumental techniques of pharmaceuticals analysis: a review,” Middle European Scientific Bulletin, vol. 45, no. 1, pp. 21-37, 2024.
- H. A. Khalil, A. F. El-Yazbi, D. A. Hamdy, and T. S. Belal, “Application of HPTLC, spectrofluorimetry and differential pulse voltammetry for determination of the antifungal drug posaconazole in suspension dosage form,” Annales Pharmaceutiques Françaises, vol. 77, no. 5, pp. 382-393, 2019.
- S. Sharma, N. Singh, A. D. Ankalgi, A. Rana, and M. S. Ashawat, “Modern trends in analytical techniques for method development and validation of pharmaceuticals: a review,” Journal of Drug Delivery & Therapeutics, vol. 11, 2021.
- H. S. AlSalem, et al., “High performance thin layer chromatography (HPTLC) analysis of anti-asthmatic combination therapy in pharmaceutical formulation: assessment of the method’s greenness and blueness,” Pharmaceuticals, vol. 17, no. 8, p. 1002, 2024.
- M. A. Bezerra, et al., “Recent developments in the application of cloud point extraction as procedure for speciation of trace elements,” Applied Spectroscopy Reviews, vol. 57, no. 4, pp. 338-352, 2022.
- E. A. Azooz, R. K. Ridha, and H. A. Abdulridha, “The fundamentals and recent applications of micellar system extraction for nanoparticles and bioactive molecules: a review,” Nano Biomed. Eng., vol. 13, no. 3, pp. 264-278, 2021.
- N. M. Muslim, B. K. Hussain, N. M. Abdulhussein, and E. A. Azooz, “Determination of selenium in black tea leaves using the air-assisted cloud point extraction method: evaluation of the method’s environmental performance,” Analytical and Bioanalytical Chemistry Research, vol. 11, no. 1, pp. 11-22, 2024.
- S. Bharti, “Recent advances in heavy metal removal from wastewater using nanomaterials and cloud point extraction: a comprehensive review,” International Journal of Environmental Science and Technology, vol. 22, no. 6, pp. 5057-5084, 2025.
- X.-W. Wei, et al., “A review on pretreatment and analysis methods of polyether antibiotics in complex samples,” Critical Reviews in Analytical Chemistry, vol. 54, no. 8, pp. 3453-3477, 2024.
- K. Oukebdane, R. Semmoud, and M. A. Didi, “Cloud point extraction of Telon Orange anionic azo-dye from aqueous sulphate solutions using Aliquat 336 ionic liquid/Tween 40 as extracting system: factorial design optimization methodology,” Desalination and Water Treatment, vol. 247, pp. 272-280, 2022.
- E. Ghasemi and M. Kaykhaii, “Application of a novel micro-cloud point extraction for preconcentration and spectrophotometric determination of azo dyes,” Journal of the Brazilian Chemical Society, vol. 27, pp. 1521-1526, 2016.
- N. A. H. Alwan and I. S. Mohammed, “Spectrophotometric determination of sulfamethazole drug by using azo coupling reaction,” Iraqi Journal for Applied Science, vol. 1, no. 1, pp. 88-101, 2024.
- A. F. Hasan and I. S. Mohammed, “Spectrophotometric determination and cloud point extraction of sulfadiazine in pure and pharmaceutical form,” Biochemical & Cellular Archives, vol. 21, no. 1, 2021.
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