Diabetes mellitus prevalence continues to rise, creating an urgent need for an effective insulin delivery mechanism through pharmacotherapy. The purpose of this research is to rigorously evaluate the efficacy of the Nano-insulin delivery system that is encapsulated in liposomes (Liposome-Insulin). This sort of Nano-system was precisely characterized using sophisticated analytical techniques like Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-Vis), and Scanning Electron Microscopy (SEM), validating its compliance to the desired range of nanosize. In this experiment, mice were used as subjects, and four groups were formed: a healthy group, an untreated diabetic group, a diabetic group treated with liposome-encapsulated Nano-insulin, and another diabetic group treated with free insulin. Measuring urea and creatinine levels meticulously assessed the renal functions. Interestingly, relevant results indicated that in the untreated diabetic mice, significant levels of renal functions were elevated, indicating hepatic and renal dysfunction. In comparison to the diabetic group administered with free insulin, there was a notable lowering in levels of urea and creatinine in the diabetic mice treated with insulin-loaded Nanoliposomes, suggesting that the insulin liposomal nanocarrier may aid in the improvement of renal functionality through significant increases in glycemic control/increased glycemic/nephroprotective agent.
Keywords
LiposomalNano-insulinKidneySEM and Histological
References
M. J. Hossain, M. Al-Mamun, and M. R. Islam, “Diabetes mellitus, the fastest growing global public health concern: Early detection should be focused,” Health Science Reports, vol. 7, no. 3, p. e2004, 2024.
L. Q. Abdulhameed et al., “Homocysteine: A recent potential risk factor for type 2 diabetes mellitus patients in Diyala Province,” in 1st Diyala International Conference for Pure and Applied Science: ICPAS2021, 2023, p. 050004.
R. Balaji, R. Duraisamy, and M. P. Kumar, “Complications of diabetes mellitus: A review,” Drug Invention Today, vol. 12, no. 1, 2019.
A. Jaffar Saleem, A. Salim Hamzah, and M. Hammadi, “Development of Sulfadiazine by Nano Method and Study Its Effect Against Multi-Drug Resistant Bacteria,” Journal of Nanostructures, vol. 14, no. 4, pp. 1023-1028, 2024, doi: 10.22052/JNS.2024.04.003.
K. Hacker, “The burden of chronic disease,” Mayo Clinic Proceedings: Innovations, Quality & Outcomes, vol. 8, no. 1, pp. 112-119, 2024.
M. Alzubaidy and M. Hussain, “Biosynthetic of green zinc oxide nanoparticles with effect on cancer cell line Hela,” Revista Bionatura, vol. 8, p. 22, 2022.
B. M. Abdul Latif and M. W. Mahdi Alzubaidy, “Effect of zinc oxide nanoparticles on activity of skin cancer cell line (A-375),” in 1st Diyala International Conference for Pure and Applied Science: ICPAS2021, 2023, p. 050003.
T. Musumeci, A. Bonaccorso, and C. Carbone, “Basic concepts of liposomes: Components, structures, properties and classification,” in Liposomes in Drug Delivery, Academic Press, 2024, pp. 19-48.
P. Liu, G. Chen, and J. Zhang, “A review of liposomes as a drug delivery system: Current status of approved products, regulatory environments, and future perspectives,” Molecules, vol. 27, no. 4, p. 1372, 2022.
D. Pasarin et al., “Coating materials to increase the stability of liposomes,” Polymers, vol. 15, no. 3, p. 782, 2023.
A. Gouda, O. S. Sakr, M. Nasr, and O. Sammour, “Ethanol injection technique for liposomes formulation: An insight into development, influencing factors, challenges and applications,” Journal of Drug Delivery Science and Technology, vol. 61, p. 102174, 2021.
M. M. Hasan et al., “Formulation development, characterization and in-vitro evaluation of tamoxifen loaded liposomes,” Journal of Pharmaceutical Research International, vol. 32, pp. 64-82, 2020.
H. Bartels and M. Bohmer, “A method for determination of serum creatinine,” Clinical Chimica Acta, vol. 32, pp. 81-85, 1971.
R. L. Searcy, J. E. Reardon, and J. A. Foreman, “A new photometric method for serum urea nitrogen determination,” American Journal of Medical Technology, vol. 33, pp. 15-20, 1967.
L. G. Luna, Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. New York, USA: McGraw-Hill, 1968.
K. S. Suvarna, C. Layton, and J. D. Bancroft, Bancroft’s Theory and Practice of Histological Techniques. Elsevier, 2018.
A. B. W. Mahdi, M. W. Mahdi, T. W. Mahdi, and R. A. S. Jassim, “Effectiveness of the Proposed Skill Exercises on Reaction Speed and Learning Skills on the Gymnastics Balance Beam for Female Students of Department of Physical Education and Sports Sciences,” Eurasian Journal of Educational Research, vol. 112, pp. 58-72, 2024, doi: 10.14689/ejer.2024.112.04.
S. Yanti, Z. W. Wu, D. C. Agrawal, and W. J. Chien, “Interaction between phloretin and insulin: A spectroscopic study,” Journal of Analytical Science and Technology, vol. 12, pp. 1-16, 2021.
L. Sercombe et al., “Advances and challenges of liposome assisted drug delivery,” Frontiers in Pharmacology, vol. 6, p. 286, 2015.
A. Akbarzadeh et al., “Liposome: Classification, preparation, and applications,” Nanoscale Research Letters, vol. 8, no. 1, p. 102, 2013.
S. U. Dandare et al., “L-alanine supplementation improves blood glucose level and biochemical indices in alloxan-induced diabetic rats,” Journal of Food Biochemistry, vol. 45, no. 1, p. e13590, 2021.
C. C. Osigwe, P. A. Akah, and C. S. Nworu, “Biochemical and haematological effects of the leaf extract of Newbouldia laevis in alloxan-induced diabetic rats,” Journal of Biosciences and Medicines, vol. 5, no. 6, pp. 18-26, 2017.
J. Häberle, B. Siri, and C. Dionisi-Vici, “Quo vadis ureagenesis disorders? A journey from 90 years ago into the future,” Journal of Inherited Metabolic Disease, vol. 47, no. 6, pp. 1120-1128, 2024.
M. Yamanouchi et al., “Retinopathy progression and the risk of end-stage kidney disease in type 2 diabetes,” BMJ Open Diabetes Research & Care, vol. 7, no. 1, p. e000726, 2019.
N. M. La-Beck and A. A. Gabizon, “Nanoparticle interactions with the immune system: Clinical implications for liposome-based cancer chemotherapy,” Frontiers in Immunology, vol. 8, p. 416, 2017.
M. G. Gonçalves et al., “Effects of creatine supplementation on histopathological and biochemical parameters in STZ-induced diabetic rats,” Nutrients, vol. 14, no. 3, p. 431, 2022.
K. Pansare et al., “Streptozotocin and alloxan-induced diabetic nephropathy: Protective role of natural products,” Journal of the Maharaja Sayajirao University of Baroda, vol. 25, 2021.
N. Yadav et al., “Assessment of prognostic markers of diabetic nephropathy: Serum creatinine and blood urea levels,” European Journal of Molecular and Clinical Medicine, vol. 7, no. 11, 2020.
M. Jovanović, D. Petrović, and S. Dinić, “Liposome encapsulation enhances the antidiabetic efficacy of silibinin,” Pharmaceutics, vol. 16, no. 6, p. 801, 2024.
R. S. Almuttairi, A. K. Shwaikh, and A. M. A. Alyassery, “The effect of repaglinide on the liver and kidney histology of alloxan-induced diabetic albino rats,” Basrah Journal of Nursing and Health Sciences, vol. 140, no. 1, pp. 1459-1466, 2022.
M. Elderbi et al., “Consequences of alloxan-induced diabetes on certain hematological and hepatic parameters in albino mice,” Khalij-Libya Journal of Dental and Medical Research, pp. 38-43, 2025.
A. H. Rahmani et al., “Quercetin attenuates diabetic complications and renal oxidative stress in STZ-induced diabetic rats,” Metabolites, vol. 13, no. 1, p. 130, 2023.
S. A. Patil and A. Jain, “Oral chitosan-encapsulated insulin nanoparticles: Effects on renal biomarkers in STZ-induced diabetic rats,” International Journal of Nanomedicine, vol. 15, pp. 1234-1245, 2020.
S. Rayego-Mateos et al., “Molecular mechanisms of kidney injury and repair,” International Journal of Molecular Sciences, vol. 23, no. 3, p. 1542, 2022.
