Green Synthesis and Cytotoxicity of CdO/Biochar Nanocomposite Against MCF-7 Cells

Neama, Ali

doi:Under indexing

Proceedings of International Conference on Applied Innovation in IT · 2026/06/12 · Vol. 14 · Issue 4 · pp. 703–710

Green Synthesis and Cytotoxicity of CdO/Biochar Nanocomposite Against MCF-7 Cells

Ali Asal Jasim and Abdulqadier Hussien Neama

📄 Download PDF DOI: Under indexing

Abstract

The growing trend in breast cancers worldwide proves the significance of the development of tough nanoparticle materials with high therapeutic potential. We demonstrate a straightforward green precipitation technique for synthesizing cadmium oxide nanoparticles utilizing cadmium nitrate as a precursor and olive leaf extract as an environmentally benign capping and stabilizing agent. Additionally, biochar obtained from household waste was utilized as a porous carbon scaffold to create a cadmium oxide/biochar binary nanocomposite, enhancing structural stability and preventing agglomeration. Characterization of Prepared Materials via X-ray diffraction, Field emission scanning microscopy, Energy-dispersive X-ray spectroscopy. The cytotoxicity (D) of pure cadmium oxide nanoparticles and (B) the cadmium oxide/biochar nanocomposite against the human breast cancer cell line (Michigan Cancer Foundation-7) as determined by MTT assay. The cadmium oxide/biochar nanocomposite (B) had the best anticancer effect, with the lowest IC₅₀ value (5.86 µg/mL), compared to pure cadmium oxide nanoparticles (D). The performance is improved because the biochar support, in combination with nanoparticles, enhances cellular uptake, leading to cancer cell death via Reactive oxygen species under near-infrared light. These findings collectively indicate that the cadmium oxide/biochar nanocomposite holds significant potential as a cost-effective and effective treatment platform for breast cancer.

Keywords

CdO Nanoparticles Biochar Green Synthesis MCF-7 Cells Breast Cancer

References

R. L. Siegel, A. N. Giaquinto, and A. Jemal, “Cancer Statistics, 2024,” CA: A Cancer Journal for Clinicians, vol. 74, no. 1, pp. 12-49, 2024.
S. Bosson-Amedenu and N. Ouerfelli, “Modeling Cancer Parameters Using GLOBOCAN World 2020 Estimates.”
Y. Liu et al., “Nanoplatform Based on Carbon Nanoparticles Loaded With Doxorubicin Enhances Apoptosis by Generating Reactive Oxygen Species for Effective Cancer Therapy,” Oncology Letters, vol. 27, no. 6, p. 288, 2024.
B. A. Ali et al., “Biosynthesis of Selenium Nanoparticles as a Potential Therapeutic Agent in Breast Cancer: G2/M Arrest and Apoptosis Induction,” Toxicology Reports, vol. 13, p. 101792, 2024.
R. Lefojane et al., “CdO/CdCO3 Nanocomposite Physical Properties and Cytotoxicity Against Selected Breast Cancer Cell Lines,” Scientific Reports, vol. 11, no. 1, p. 30, 2021.
M. N. Abu Hajleh et al., “Synergistic Effects of AgNPs and Biochar: A Potential Combination for Combating Lung Cancer and Pathogenic Bacteria,” Molecules, vol. 28, no. 12, p. 4757, 2023.
S. A. Bhat et al., “Biogenic Nanoparticles: Pioneering a New Era in Breast Cancer Therapeutics - A Comprehensive Review,” Discover Nano, vol. 19, no. 1, p. 121, 2024.
Z. T. Ibrahim, Z. T. Alkayar, A. M. Rashed, G. A. Atiya, and A. H. Al Khazraji, “Preparation of CdO Nanoparticles Using Two Different Salts and Investigation of Their Antibacterial Activity,” in Proceedings of International Conference on Applied Innovation in IT, vol. 13, no. 2, Anhalt University of Applied Sciences, 2025, pp. 565-570.
R. Lefojane et al., “CdO/CdCO3 Nanocomposite Physical Properties and Cytotoxicity Against Selected Breast Cancer Cell Lines,” Scientific Reports, vol. 11, no. 1, p. 30, 2021.
M. Zahera et al., “Cadmium Oxide Nanoparticles: An Attractive Candidate for Novel Therapeutic Approaches,” Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 585, p. 124017, 2020.
M. N. Abu Hajleh et al., “Synergistic Effects of AgNPs and Biochar: A Potential Combination for Combating Lung Cancer and Pathogenic Bacteria,” Molecules, vol. 28, no. 12, p. 4757, 2023.
A. J. J. Makkawi, N. H. Aysa, and F.-A. G. Gassim, “Anticancer Activity of Zinc Oxide and Zinc Oxide/Cadmium Sulfide Nanocomposites,” Asian Journal of Pharmaceutical and Clinical Research, pp. 535-539, 2019.
A. J. Saleem, A. Salim Hamzah, and M. Hammadi, “Development of Sulfadiazine by Nano Method and Study Its Effect Against Multi-Drug Resistant Bacteria,” Journal of Nanostructures, vol. 14, no. 4, pp. 1023-1028, 2024, [Online]. Available: https://doi.org/10.22052/JNS.2024.04.003.
P. Rajabzadeh, P. Zoorazma, M. Abbasi, and S. A. Sadat Shandiz, “Preparation of Iron Oxide/Palladium Nanoparticles Modified With Carbon Quantum Dots (Pd@CQD@Fe3O4): Insight to Anti-Cancer Effect and ROS Generation,” Scientific Reports, vol. 15, no. 1, p. 17317, 2025.
E. A. Diab, M. Ghali, and M. Mosaad, “In Vitro Antimicrobial and Anticancer Potentials of Green Synthesized Luminescent Carbon Quantum Dots Derived From Artichoke Leaves,” Scientific Reports, vol. 15, no. 1, p. 16199, 2025.
A. D. Bidgoli, A. Farmany, M. Taheri, M. Soleimani, and F. Nouri, “Preparation and Characterization of Calcium-Doped Graphene Oxide-Chitosan Nanocarrier to Enhance the Gene Delivery in MCF-7 Cell Line,” Scientific Reports, vol. 14, no. 1, p. 27434, 2024.
A. Mazhar, N. S. Elkholy, N. Yousif, and M. Shalaby, “Investigations of Structural, Optical, Thermal, and Spectroscopic Characteristics of CdO-Ni Nanoparticles Employed in Anti-Cancer Activities for Cancer Cells,” Results in Chemistry, vol. 6, p. 101204, 2023.
B. A. Ali, R. M. Allam, M. S. Hasanin, and A. A. Hassabo, “Biosynthesis of Selenium Nanoparticles as a Potential Therapeutic Agent in Breast Cancer: G2/M Arrest and Apoptosis Induction,” Toxicology Reports, vol. 13, p. 101792, 2024.
F. Motafeghi, M. Gerami, P. Mortazavi, B. Khayambashi, N. Ghassemi-Barghi, and M. Shokrzadeh, “Green Synthesis of Silver Nanoparticles, Graphene, and Silver-Graphene Nanocomposite Using Melissa officinalis Ethanolic Extract: Anticancer Effect on MCF-7 Cell Line,” Iranian Journal of Basic Medical Sciences, vol. 26, no. 1, p. 57, 2023.
M. Saravanan et al., “Emerging Antineoplastic Biogenic Gold Nanomaterials for Breast Cancer Therapeutics: A Systematic Review,” International Journal of Nanomedicine, pp. 3577-3595, 2020.
M. B. Aboul-Nasr, A. A. Yasien, S. S. Mohamed, Y. B. Aboul-Nasr, and M. Obiedallah, “Exploring the Anticancer Potential of Green Silver Nanoparticles-Paclitaxel Nanocarrier on MCF-7 Breast Cancer Cells: An In Vitro Approach,” Scientific Reports, vol. 15, no. 1, p. 20198, 2025.
M. V. Shestovskaya, A. L. Luss, O. A. Bezborodova, V. V. Makarov, and A. A. Keskinov, “Iron Oxide Nanoparticles in Cancer Treatment: Cell Responses and the Potency to Improve Radiosensitivity,” Pharmaceutics, vol. 15, no. 10, p. 2406, 2023.
M. M. El-Sheekh, E.-R. Kenawy, W. E. Yousuf, and T. M. Mohamed, “Inhibition of Malate Dehydrogenase via Nanoselenium Coupling With Nanocellulose Composite in MCF-7 Breast Carcinoma Cells,” Cancer Nanotechnology, vol. 15, no. 1, p. 52, 2024.
T. Tunç, C. Hepokur, and A. Kari̇per, “Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity,” Bioinorganic Chemistry and Applications, vol. 2024, no. 1, p. 9916187, 2024.
N. Seifi et al., “Anti-Cancerous Effect and Biological Evaluation of Green Synthesized Selenium Nanoparticles on MCF-7 Breast Cancer and HUVEC Cell Lines,” Nanomedicine Research Journal, vol. 8, no. 4, pp. 373-382, 2023.
N. Shanmugam, B. Saravanan, R. Reagan, N. Kannadasan, K. Sathishkumar, and S. Cholan, “Effect of Thermal Annealing on the Cd(OH)2 and Preparation of CdO Nanocrystals,” Modern Chemistry & Applications, vol. 2, pp. 1-5, 2014.
M. A. Hamzah, A. H. Alwan, and S. S. Hussain, “In-Vitro Cytotoxic Activity of E. coli Outer Membrane Vesicles (OMVs) Against Breast Cancer (MCF-7) Cell Line,” Prof. (Dr.) R. K. Sharma, vol. 21, no. 1, p. 474, 2021.